Human Thrombopoietin Quantikine ELISA Kit
Human Thrombopoietin Quantikine ELISA Kit
Human Thrombopoietin Quantikine ELISA Kit Summary
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貨號：DTP00B
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Tpo
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Tpo Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Tpo in cell culture supernates, serum, and plasma. It contains recombinant human Tpo and antibodies raised against the recombinant factor. Results obtained using natural human Tpo showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Tpo.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
194
|
512
|
983
|
187
|
517
|
970
|
|
Standard Deviation
|
17.5
|
16.5
|
26.8
|
16.3
|
39.2
|
63.9
|
|
CV%
|
9
|
3.2
|
2.7
|
8.7
|
7.6
|
6.6
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
204
|
579
|
1140
|
203
|
555
|
1070
|
|
Standard Deviation
|
13.7
|
15.1
|
52.6
|
19.3
|
48.7
|
92.8
|
|
CV%
|
6.7
|
2.6
|
4.6
|
9.5
|
8.8
|
8.7
|
Recovery
The recovery of Tpo spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
99
|
89-109
|
|
Platelet-poor EDTA Plasma (n=4)
|
95
|
85-102
|
|
Platelet-poor Heparin Plasma (n=4)
|
93
|
86-106
|
|
Serum (n=4)
|
102
|
90-111
|
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Tpo were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
