Human Total MMP-3 Quantikine ELISA Kit
Human Total MMP-3 Quantikine ELISA Kit
Human Total MMP-3 Quantikine ELISA Kit Summary
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貨號：DMP300
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant human total MMP-3
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Total MMP-3 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure total MMP-3 (pro- and active MMP-3) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant human active MMP-3. Natural human MMP-3 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.962
|
3.21
|
5.87
|
0.994
|
3.08
|
5.7
|
|
Standard Deviation
|
0.062
|
0.197
|
0.332
|
0.085
|
0.217
|
0.449
|
|
CV%
|
6.4
|
6.1
|
5.7
|
8.6
|
7
|
7.9
|
Recovery
The recovery of MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
96
|
87-104
|
|
Heparin Plasma (n=5)
|
92
|
85-106
|
|
Serum (n=5)
|
90
|
86-96
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of MMP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Data Examples
