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          Human VEGF-D Quantikine ELISA Kit


          

          
Human VEGF-D Quantikine ELISA Kit
          Human VEGF-D Quantikine ELISA Kit
          
            Human VEGF-D Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	
          

          

          
	Human VEGF-D Quantikine ELISA Kit Summary

          

          
	
          

          

          
	貨號：DVED00

          

          
	深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！

          

          
	
          
		
          
			
          
				
          
					Assay Type
				
          
				
          
					Solid Phase Sandwich ELISA
				
          
			
          
			
          
				
          
					Format
				
          
				
          
					96-well strip plate
				
          
			
          
			
          
				
          
					Assay Length
				
          
				
          
					4.5 hours
				
          
			
          
			
          
				
          
					Sample Type & Volume Required Per Well
				
          
				
          
					Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
				
          
			
          
			
          
				
          
					Sensitivity
				
          
				
          
					31.3 pg/mL
				
          
			
          
			
          
				
          
					Assay Range
				
          
				
          
					125.0 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
				
          
			
          
			
          
				
          
					Specificity
				
          
				
          
					Natural and recombinant human VEGF-D
				
          
			
          
			
          
				
          
					Cross-reactivity
				
          
				
          
					< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
				
          
			
          
			
          
				
          
					Interference
				
          
				
          
					No significant interference observed with available related molecules.
				
          
			
          
		
          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Human VEGF-D Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF-D levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed, recombinant human VEGF-D and antibodies raised against the recombinant protein. Results obtained for naturally occurring human VEGF-D showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF-D.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Cell Culture Supernates
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					40
				
				
					40
				
				
					40
				
			
          
			
          
				
					Mean
				
				
					560
				
				
					1098
				
				
					2192
				
				
					512
				
				
					978
				
				
					1978
				
			
          
			
          
				
					Standard Deviation
				
				
					31.8
				
				
					35.5
				
				
					45.6
				
				
					46.3
				
				
					72.8
				
				
					117
				
			
          
			
          
				
					CV%
				
				
					5.7
				
				
					3.2
				
				
					2.1
				
				
					9
				
				
					7.4
				
				
					5.9
				
			
          
		          
	
          

          
	
          
		Serum, EDTA Plasma, Heparin Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					40
				
				
					40
				
				
					40
				
			
          
			
          
				
					Mean
				
				
					448
				
				
					970
				
				
					2081
				
				
					474
				
				
					956
				
				
					2013
				
			
          
			
          
				
					Standard Deviation
				
				
					27.8
				
				
					40.9
				
				
					49.3
				
				
					38
				
				
					68.5
				
				
					145
				
			
          
			
          
				
					CV%
				
				
					6.2
				
				
					4.2
				
				
					2.4
				
				
					8
				
				
					7.2
				
				
					7.2
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of VEGF-D spiked to three different levels throughout the range of the assay in various matrices was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=4)
				
				
					100
				
				
					97-104
				
			
          
			
          
				
					EDTA Plasma (n=5)
				
				
					105
				
				
					89-114
				
			
          
			
          
				
					Heparin Plasma (n=5)
				
				
					104
				
				
					88-114
				
			
          
			
          
				
					Serum (n=5)
				
				
					106
				
				
					94-113
				
			
          
		          
	
          

          

          

          
	
          

          

          
	DVED00

          

          
	
          
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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