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          Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit


          

          
Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit
          Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit
          
            Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
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	Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit Summary

          

          
	貨號：DCDL40

          

          
	規(guī)格：96T

          

          
	
          
		
          
			
          
				Assay Type
			
          
			
          
				Solid Phase Sandwich ELISA
			
          
		
          
		
          
			
          
				Format
			
          
			
          
				96-well strip plate
			
          
		
          
		
          
			
          
				Assay Length
			
          
			
          
				4.5 hours
			
          
		
          
		
          
			
          
				Sample Type & Volume Required Per Well
			
          
			
          
				Cell Culture Supernates (100 uL), Serum (20 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
			
          
		
          
		
          
			
          
				Sensitivity
			
          
			
          
				10.1 pg/mL
			
          
		
          
		
          
			
          
				Assay Range
			
          
			
          
				62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
			
          
		
          
		
          
			
          
				Specificity
			
          
			
          
				Natural and recombinant human CD40 Ligand
			
          
		
          
		
          
			
          
				Cross-reactivity
			
          
			
          
				< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
			
          
		
          
		
          
			
          
				Interference
			
          
			
          
				No significant interference observed with available related molecules.
			
          
		
          
	
          

          

          
	
          

          

          
	
          
		Product Summary
	
          
	
          
		The Quantikine Human CD40 Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CD40 Ligand in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CD40 Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD40 Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD40 Ligand.
	
          

          

          
	
          
		Precision
	
          
	
          
		Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
	
          
	
          
		Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
	
          
	
          
		Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
	
          
	
          
		
			
          
				
				
				
					Intra-Assay Precision
				
				
					Inter-Assay Precision
				
			
          
		          
		
			
          
				
					Sample
				
				
					1
				
				
					2
				
				
					3
				
				
					1
				
				
					2
				
				
					3
				
			
          
			
          
				
					n
				
				
					20
				
				
					20
				
				
					20
				
				
					40
				
				
					40
				
				
					40
				
			
          
			
          
				
					Mean
				
				
					430
				
				
					1212
				
				
					2638
				
				
					437
				
				
					1205
				
				
					2612
				
			
          
			
          
				
					Standard Deviation
				
				
					21.8
				
				
					54.4
				
				
					143
				
				
					27.9
				
				
					72.7
				
				
					162
				
			
          
			
          
				
					CV%
				
				
					5.1
				
				
					4.5
				
				
					5.4
				
				
					6.4
				
				
					6
				
				
					6.2
				
			
          
		          
	
          

          

          

          
	
          
		Recovery
	
          
	
          
		
          
			The recovery of CD40 Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.
		
          
	
          
	
          
		
			
          
				
					Sample Type
				
				
					Average % Recovery
				
				
					Range %
				
			
          
		          
		
			
          
				
					Cell Culture Media (n=4)
				
				
					102
				
				
					90-114
				
			
          
			
          
				
					Platelet-poor EDTA Plasma (n=5)
				
				
					98
				
				
					86-107
				
			
          
			
          
				
					Platelet-poor Heparin Plasma (n=5)
				
				
					94
				
				
					86-113
				
			
          
		          
	
          

          

          

          
	
          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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