Human Myeloperoxidase Quantikine ELISA Kit
Human Myeloperoxidase Quantikine ELISA Kit
Human Myeloperoxidase Quantikine ELISA Kit Summary
貨號：DMYE00B
規(guī)格：96T
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Urine)
Specificity
Natural human MPO
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MPO Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MPO in cell culture supernates, cell lysates, serum, platelet-poor plasma, saliva, and urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
0.994
|
3.36
|
6.68
|
0.974
|
2.99
|
6.23
|
|
Standard Deviation
|
0.034
|
0.085
|
0.123
|
0.078
|
0.18
|
0.38
|
|
CV%
|
3.4
|
2.5
|
1.8
|
8
|
6
|
6
|
Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
1.03
|
3.13
|
6.63
|
1.07
|
3.29
|
6.71
|
|
Standard Deviation
|
0.024
|
0.046
|
0.172
|
0.12
|
0.26
|
0.55
|
|
CV%
|
2.3
|
1.5
|
2.6
|
10.8
|
8
|
8.2
|
