Mouse CCL11/Eotaxin Quantikine ELISA Kit
Mouse CCL11/Eotaxin Quantikine ELISA Kit
Mouse CCL11/Eotaxin Quantikine ELISA Kit Summary
貨號：MME00
規(guī)格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Citrate Plasma (13 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant mouse Eotaxin. This assay cross-reacts 0.062% with Human Eotaxin and recognizes Rat Eotaxin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Eotaxin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Eotaxin in cell culture supernates, serum, and plasma. It contains recombinant mouse Eotaxin and antibodies raised against the recombinant protein. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse Eotaxin showed dose curves that were parallel to the standard curves obtained using the E. coli-expressed kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Eotaxin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
49
|
197
|
466
|
46
|
179
|
446
|
|
Standard Deviation
|
3.2
|
7.2
|
30
|
3.8
|
11.7
|
28.7
|
|
CV%
|
6.5
|
3.7
|
6.4
|
8.3
|
6.5
|
6.4
|
Recovery
The recovery of mouse Eotaxin spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
96
|
82-103
|
|
Citrate Plasma (n=5)
|
97
|
81-114
|
|
EDTA Plasma (n=5)
|
100
|
83-118
|
|
Serum (n=5)
|
98
|
82-111
|
Linearity
