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          Human VEGF-C Quantikine ELISA Kit
          Human VEGF-C Quantikine ELISA Kit
          Human VEGF-C Quantikine ELISA Kit

          Human VEGF-C Quantikine ELISA Kit

           詳情說明


          Human VEGF-C Quantikine ELISA Kit Summary


          貨號:DVEC00

          規(guī)格:96T

          深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒,價格優(yōu)勢,貨期短,歡迎來電咨詢0755-33164177!

          Assay Type
          Solid Phase Sandwich ELISA
          Format
          96-well strip plate
          Assay Length
          4.5 hours
          Sample Type & Volume Required Per Well
          Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
          Sensitivity
          48.4 pg/mL
          Assay Range
          109.0 - 7,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma, Saliva)
          Specificity
          Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)
          Cross-reactivity
          < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
          Interference
          No significant interference observed with available related molecules.

          Product Summary

          The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.

          Precision

          Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
          Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

          Cell Culture Supernates, Saliva

          Intra-Assay Precision Inter-Assay Precision
          Sample 1 2 3 1 2 3
          n 20 20 20 40 40 40
          Mean 572 1622 3243 520 1527 3069
          Standard Deviation 39.3 54.4 136 50.1 102 200
          CV% 6.9 3.4 4.2 9.6 6.7 6.5

          Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma

          Intra-Assay Precision Inter-Assay Precision
          Sample 1 2 3 1 2 3
          n 20 20 20 40 40 40
          Mean 512 1543 3195 514 1540 3066
          Standard Deviation 33.8 54.2 136 43.6 110 198
          CV% 6.6 3.5 4.3 8.5 7.2 6.4

          Recovery

          The recovery of VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated.

          Sample Type Average % Recovery Range %
          Cell Culture Supernates (n=4) 107 93-115
          EDTA Plasma (n=4) 101 96-110
          Heparin Plasma (n=4) 97 88-103
          Serum (n=4) 99 89-106


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