熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統一客服熱線：
0755-28715175
熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統一客服熱線：
0755-28715175
深圳子科生物優(yōu)勢代理美國R&DELISA系列產品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
貨號：MOP00
規(guī)格：96T
Cell Culture Supernates, Serum, EDTA Plasma
The recovery of mouse OPG spiked to three levels throughout the range of the assay in various matrices was evaluated.
Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
Sensitivity
6.9 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse OPG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse OPG Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse OPG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse OPG and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse OPG accurately. Results obtained using natural mouse OPG showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse OPG.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
69.9
311
650
68
332
741
Standard Deviation
5.5
13.4
35.6
5
23
54
CV%
7.9
4.3
5.5
7.4
6.9
7.3
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=9)
101
85-116
EDTA Plasma (n=9)
97
88-107
Serum (n=11)
93
83-108
Linearity
To assess the linearity of the assay, five or more samples containing various concentrations of OPG in each matrix were diluted with Calibrator Diluent and then assayed.
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