熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
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貨號：MBLYS0
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
The recovery of mouse BAFF/BLyS spiked to three levels throughout the range of the assay was evaluated.
Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (40 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL)
Sensitivity
7.8 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse BAFF/BLyS
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse BAFF/BLyS and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse BAFF/BLyS.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
50
64
56
Mean
125
288
1315
127
325
1262
Standard Deviation
3.6
7
30.3
16
27
136
CV%
2.9
2.4
2.3
12.6
8.3
10.8
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=6)
103
90-108
EDTA Plasma (n=6)
96
85-103
Heparin Plasma (n=6)
94
85-100
Serum (n=6)
98
86-115
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