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          Mouse Thrombopoietin Quantikine ELISA Kit


          

          
Mouse Thrombopoietin Quantikine ELISA Kit
          Mouse Thrombopoietin Quantikine ELISA Kit
          
            Mouse Thrombopoietin Quantikine ELISA Kit
          
            
           詳情說明
          
                
          
	深圳子科生物優(yōu)勢(shì)代理美國(guó)R&DELISA系列產(chǎn)品試劑盒，價(jià)格優(yōu)勢(shì)，貨期短，歡迎來電咨詢0755-33164177！

          

          
	
          
		
          
			Mouse Thrombopoietin Quantikine ELISA Kit Summary
		
          
		
          
			貨號(hào)：MTP00
		
          
		
          
			
          
				
          
					
          
						Assay Type
					
          
					
          
						Solid Phase Sandwich ELISA
					
          
				
          
				
          
					
          
						Format
					
          
					
          
						96-well strip plate
					
          
				
          
				
          
					
          
						Assay Length
					
          
					
          
						4.5 hours
					
          
				
          
				
          
					
          
						Sample Type & Volume Required Per Well
					
          
					
          
						Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Citrate Plasma (10 uL)
					
          
				
          
				
          
					
          
						Sensitivity
					
          
					
          
						20 pg/mL
					
          
				
          
				
          
					
          
						Assay Range
					
          
					
          
						62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
					
          
				
          
				
          
					
          
						Specificity
					
          
					
          
						Natural and recombinant mouse Tpo
					
          
				
          
				
          
					
          
						Cross-reactivity
					
          
					
          
						< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
					
          
				
          
				
          
					
          
						Interference
					
          
					
          
						No significant interference observed with available related molecules.
					
          
				
          
			
          
		
          
	
          
	
          
		
          
			Product Summary
		
          
		
          
			The Quantikine Mouse Tpo Immunoassay is a 4.5 hour solid phase ELISA designed to measure Tpo in cell culture supernates, serum, and plasma. It contains recombinant mouse Tpo and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Tpo showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Tpo.
		
          
	
          
	
          
		
          
			Precision
		
          
		
          
			Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
		
          
		
          
			Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
		
          
		
          
			Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma
		
          
		
          
			
				
          
					
					
					
						Intra-Assay Precision
					
					
						Inter-Assay Precision
					
				
          
			          
			
				
          
					
						Sample
					
					
						1
					
					
						2
					
					
						3
					
					
						1
					
					
						2
					
					
						3
					
				
          
				
          
					
						n
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
					
						20
					
				
          
				
          
					
						Mean
					
					
						123
					
					
						455
					
					
						2364
					
					
						119
					
					
						457
					
					
						2389
					
				
          
				
          
					
						Standard Deviation
					
					
						9.8
					
					
						17
					
					
						58.4
					
					
						9.6
					
					
						21.7
					
					
						115
					
				
          
				
          
					
						CV%
					
					
						8
					
					
						3.7
					
					
						2.5
					
					
						8.1
					
					
						4.7
					
					
						4.8
					
				
          
			          
		
          

          
	
          
	
          
		
          
			Recovery
		
          
		
          
			
          
				The recovery of mouse Tpo spiked to three levels throughout the range of the assay in various matrices was evaluated.
			
          
		
          
		
          
			
				
          
					
						Sample Type
					
					
						Average % Recovery
					
					
						Range %
					
				
          
			          
			
				
          
					
						Cell Culture Media (n=5)
					
					
						97
					
					
						87-115
					
				
          
				
          
					
						Citrate Plasma (n=5)
					
					
						104
					
					
						88-113
					
				
          
				
          
					
						EDTA Plasma (n=5)
					
					
						104
					
					
						87-116
					
				
          
				
          
					
						Serum (n=5)
					
					
						100
					
					
						88-111
					
				
          
			          
		
          
	
          

          

          
		
          
    
	
          

          


          

          

          

          

          

          


          
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