Rat IFN-gamma Quantikine ELISA Kit
Rat IFN-gamma Quantikine ELISA Kit
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Rat IFN-gamma Quantikine ELISA Kit Summary
Catalog Size / Price
RIF00 1 Kit / Please Inquire
SRIF00 1 Pack (6 Plates) / Please Inquire
PRIF00 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Specificity
Natural and recombinant rat IFN-gamma
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IFN-gamma levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant rat IFN-gamma accurately. Results obtained using natural rat IFN-gamma showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IFN-gamma.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
86.9
|
215
|
1159
|
82.8
|
205
|
1135
|
|
Standard Deviation
|
3.5
|
4.4
|
23
|
8
|
14.5
|
48
|
|
CV%
|
4
|
2
|
2
|
9.7
|
7.1
|
4.2
|
Recovery
The recovery of rat IFN-gamma spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
108
|
89-119
|
|
Serum (n=6)
|
100
|
89-111
|
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of rat IFN-gamma in each matrix were diluted with Calibrator Diluent and then assayed.
