熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
貨號：DTRM10C 規(guī)格:96T
深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva
The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Sensitivity
15.2 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human TREM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human TREM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TREM-1 levels in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TREM-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human TREM-1 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human TREM-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
479
1226
2217
511
1286
2254
Standard Deviation
16.8
41.5
72.1
25
57.7
90.7
CV%
3.5
3.4
3.3
4.9
4.5
4
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
102
94-112
Cell Lysis Buffer (n=4)
88
78-96
EDTA Plasma (n=4)
99
92-105
Heparin Plasma (n=4)
96
87-104
Serum (n=4)
97
84-107
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human TREM-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
0755-28715175/33164177
粵公網(wǎng)安備 44030902000304號
