Human FGF basic Quantikine HS ELISA Kit
Human FGF basic Quantikine HS ELISA Kit
Human FGF basic Quantikine HS ELISA Kit Summary
Catalog Size / Price
HSFB00D 1 Kit / Please Inquire
SSFB00D 1 Pack (6 Plates) / Please Inquire
PHSFB00D 1 Pack (50 Plates) / Please Inquire
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Assay Range
0.3 - 20 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human FGF basic
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine HS Human FGF basic Immunoassay kit is a 5.5 hour solid phase ELISA designed to measure FGF basic levels in serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1.31
|
5.32
|
11.4
|
1.51
|
5.71
|
11.1
|
|
Standard Deviation
|
0.1
|
0.23
|
0.4
|
0.12
|
0.27
|
0.91
|
|
CV%
|
7.7
|
4.4
|
3.5
|
8.2
|
4.7
|
8.2
|
Recovery
The recovery of FGF basic spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Citrate Plasma (n=4)
|
97
|
83-118
|
|
EDTA Plasma (n=4)
|
99
|
85-109
|
|
Serum (n=4)
|
100
|
88-116
|
|
Urine (n=4)
|
109
|
100-120
|
Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and assayed.
