Mouse/Rat Flt-3 Ligand Quantikine ELISA Kit
Mouse/Rat Flt-3 Ligand Quantikine ELISA Kit
Mouse/Rat Flt-3 Ligand Quantikine ELISA Kit Summary
貨號：MFK00
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse Flt-3 Ligand and natural rat Flt-3 Ligand
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat Flt-3 Ligand Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat Flt-3 Ligand levels in cell culture supernates and serum. It contains NS0-expressed recombinant mouse Flt-3 Ligand and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse or rat Flt-3 Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse/rat Flt-3 Ligand.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
79.5
|
237
|
775
|
82.4
|
242
|
768
|
|
Standard Deviation
|
3.9
|
10.1
|
28.6
|
6.2
|
13.6
|
39.4
|
|
CV%
|
4.9
|
4.3
|
3.7
|
7.5
|
5.6
|
5.1
|
Recovery
The recovery of mouse Flt-3 Ligand spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=5)
|
99
|
88-107
|
|
Mouse Serum (n=5)
|
95
|
82-106
|
|
Rat Cell Culture Supernates (n=5)
|
108
|
99-119
|
|
Rat Serum (n=5)
|
95
|
86-114
|
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Flt-3 Ligand in each matrix were diluted with Calibrator Diluent and then assayed.
