Human Prolactin Quantikine ELISA Kit
Human Prolactin Quantikine ELISA Kit
深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
Human Prolactin Quantikine ELISA Kit Summary
貨號：DPRL00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
1.6 - 100 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Prolactin and natural human macroprolactin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human Prolactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Prolactin in serum and plasma. It contains E. coli-expressed recombinant human Prolactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Prolactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Prolactin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to
assess inter-assay precision. Assays were performed by at least three
technicians using two lots of kit components
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
16.3
|
31.8
|
53.1
|
15.4
|
30.6
|
54.4
|
|
Standard Deviation
|
0.831
|
1.24
|
1.79
|
0.9
|
1.48
|
2.94
|
|
CV%
|
5.1
|
3.9
|
3.4
|
5.8
|
4.8
|
5.4
|
Recovery
The recovery of Prolactin spiked to levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
EDTA Plasma (n=4)
|
94
|
84-105
|
|
Heparin Plasma (n=4)
|
97
|
90-104
|
|
Serum (n=4)
|
94
|
84-108
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Prolactin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
：DPRL00DPRL00
