Human IGF-II Quantikine ELISA Kit
Human IGF-II Quantikine ELISA Kit
Human IGF-II Quantikine ELISA Kit Summary
貨號：DG200
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Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)
Assay Range
12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IGF-II.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
128
|
305
|
566
|
136
|
307
|
558
|
|
Standard Deviation
|
4.05
|
7.2
|
15.6
|
9.89
|
28.5
|
28.2
|
|
CV%
|
3.2
|
2.4
|
2.8
|
7.3
|
9.3
|
5.1
|
Recovery
The recovery of human IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
|
Serum-free Cell Culture Media (n=4)
|
95
|
82-104
|
|
Urine (n=4)
|
94
|
79-104
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
