Human IL-18 BPa Quantikine ELISA Kit
Human IL-18 BPa Quantikine ELISA Kit
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Human IL-18 BPa Quantikine ELISA Kit Summary
貨號：DBP180
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Assay Range
26.6 - 1,700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IL-18 BPa
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-18 BPa in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-18 BPa. Results obtained using natural human IL-18 BPa showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 BPa.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
109
|
368
|
740
|
115
|
362
|
692
|
|
Standard Deviation
|
5.73
|
16.4
|
15.8
|
13.5
|
29.1
|
48.3
|
|
CV%
|
5.3
|
4.5
|
2.1
|
11.7
|
8
|
7
|
Cell Culture Supernates, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
123
|
404
|
789
|
136
|
434
|
841
|
|
Standard Deviation
|
5.5
|
9.49
|
21.1
|
9.47
|
32.8
|
59.1
|
|
CV%
|
4.5
|
2.3
|
2.7
|
7
|
7.6
|
7
|
Recovery
The recovery of human IL-18 BPa spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
106
|
98-114
|
|
EDTA Plasma (n=4)
|
106
|
93-113
|
|
Heparin Plasma (n=4)
|
105
|
92-119
|
|
Serum (n=4)
|
102
|
92-113
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
