Human BMP-4 Quantikine ELISA Kit
Human BMP-4 Quantikine ELISA Kit
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Human BMP-4 Quantikine ELISA Kit Summary
貨號：DBP400
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Bone Extracts (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Bone Extracts)
Specificity
Natural and recombinant human BMP-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human BMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure BMP-4 levels in bone tissue extracts and cell culture supernates. It contains NS0-expressed recombinant human BMP-4 and antibodies raised against the recombinant factor.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Bone Extracts
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
301
|
719
|
1351
|
289
|
734
|
1429
|
|
Standard Deviation
|
15.9
|
22.5
|
60.1
|
16.9
|
42.7
|
75.4
|
|
CV%
|
5.3
|
3.1
|
4.4
|
5.8
|
5.8
|
5.3
|
Recovery
The recovery of recombinant human BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
2 M Guanidine-HCI (n=2)
|
96
|
93-103
|
|
2 M Urea (n=2)
|
97
|
91-105
|
|
Cell Culture Media (n=8)
|
100
|
91-109
|
Linearity
The following samples were spiked with rhBMP-4 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess linearity of the assay.
